PG Preparation

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Stains for Organisms


a) Pneumococci b) Cryptococci	India ink India ink
C. Diptheriae	Methylene Blue, Ponder’s , Albert’s, Neisser’s Stains
Treponemes	a) Giemsa Stain b) Levaditi Stain
Anthrax	Sudan black
Gr. Inguinale	Wright’s Stain
Pneumocystisis carinii	Methanamine silver nitrate
Trichomonas	Brilliant Cresylviolet 1%
Nocardia	Acid fast stains
T.B	Auramine and Rhodamine also
Nocardia, TB, Leprosy	Acid fast stains.

Arrangement of Bacteria

Streptococci Chains

Anthrax Chains ( Bamboo stick appearance)

Staphylococci Bunches

Pneumococci Pairs

Enterococci Pairs

Meningococci Pairs mainly intracellular

Gonococci Pairs mainly intracellular

Diptheriae Cuneiform or Chinese letter pattern

Enterococci Pairs at angle ( Oval Cells) or short chains

Cl. Welchii Single or chain or bundles

Morax- Axenfield Bacilus Diplobacillus

Leprosy Bacilli In globi, cigar bundle appearance