PG Preparation
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Selective
Media for Organisms
|
Organism
|
Medium
|
| Pseudomonas |
Cetrimide
agar |
| Gonorrhoea |
Thayer Martin
and Chacko Nair egg enriched medium. |
| Bordetella
Pertussis |
Bordet- Genghou
medium |
| Brucella |
Castaneda
agar |
| T.B. Bacillus |
Lowenstein-
Jensen’s Medium. |
| Borrelia |
Noguchi’s
medium |
| Corynebacterium |
Loeffler’s
medium |
| Staphylococci |
8- 10 % Nacl
Tellurite medium in salt broth |
| Shigella |
Deoxycholate-
Citrate agar |
| Clostridia |
Robertson’s
cooked meat broth, Rabbitserum ( Fletcher Stuarts Medium) |
| Leptospirosis |
Chorio- Allantoic
membrane of Chick embryo |
| Anti
body sensitivity test |
1.
Nutrient agar 2. Muller Hinton agar |
| Transport
of Streptococci |
Pikes medium |
| Transport
of cholera organisms |
a)
Venkatraman- Ramakrishnan Medium b)Cary- Blair Medium |
| Plating
of Cholera organisms |
a)
Alkaline Bile salt agar b) G.T.T.A medium c) T.C.B.S medium |
| Salmonella |
Bile
broth , Wilson Blair Medium, Selenite – F and Tetrathionate |
| Anthrax |
Polymixin,
Lysozome, EDTA, Thallous Acetate ( PLET) |
| Meningococci |
Muller Hinton
Agar |
| Candida |
Sabouraud’s
medium |
Dark ground Illumination helps to
detect
1. Leptospirosis 2. Treponemes 3.
Borrelia
All cocci are Gram positive except
Neisseria
All Bacilli are Gram Negative except
D- Diptheria, A- Actinomycosis, T- Tetani Cl, T- Tuberculosis Myco, A-
Anthrax (DATTA)
Generation time of:
| Lepra Bacillus |
20 days roughly |
| Tuberculosis
Bacillus |
20 Hours |
| Coliforms |
20 minutes |
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|
